Technical Information and general usage instructions
Properties of Menzel glass
 | Borosilicate glass D263 (pdf, 14kb) |
| Crownglass Microcrown (pdf, 14kb) |
| Extra-white Electroverre-Glass (pdf, 32kb) |
(pdf) To view these files you will need to have Adobe Acrobat Reader.
You can download a free copy from this link: 
Storage and handling
| Storage and handling instructions |
| SuperFrost® usage instruction |
| Polysine™ usage instruction |
| SuperFrost® Plus GOLD usage instruction |
| SuperFrost Ultra Plus® usage instruction |
DNA Array und Hybridization protocols
| DNA Array protocol for SuperChip™ |
| Hybridization protocol |
You will find the protocols and further information here:http://www.eriemicroarray.com/support/tech.aspx
Storage and Usage Instructions
| Slides and cover glasses are particularly suitable for the microscopic analysis of blood, urine and tissue samples. |
| Slides and cover glasses are ready to use and should not be reused. |
| Be aware of the possibility of harm when dealing with the slides and cover glasses, and take the corresponding safety measures, e.g. wearing gloves. |
| When using the slides and cover glasses in machines, the additional usage instructions from the manufacturer of the machinery should be followed. |
| When using dyes or other chemicals, please note the safety advice from the manufacturer. |
| Storage: |
- Keep dry.
- Avoid large variations in temperature during both storage and usage. Cooling of the product can cause condensation and lead to condensed water forming between the glasses.
- Please note: Use older products in storage first.
- Protect the bottom of the carton from dampness.
- Slide storage periods: see "use before" date.
| If you have any questions concerning our products, please contact us. |
Stand: 21.10.2002
SuperFrost® Plus Instructions For Use
| Float your 2 to 5 micron thick tissue sections on a pre-heated distilled water bath. Do not add glue or subbing solution to the bath. The SuperFrost® Plus slide pre-treatment eliminates the need for these compounds. (Note: You may try substituting tap water for distilled water. However, if you begin losing tissue sections, use only distilled water.) |
| Mount your tissue sections carefully. Tissue bonding begins quickly on SuperFrost® Plus slides. |
| Dry slides completely at room temperature by vertically draining them before heating them in an oven. |
Polysine™ Instructions For Use
| Float 2 to 5 micron thick tissue sections on a pre-heated distilled water bath. Do not add glue or subbing solution to the bath. Polysine™ slides eliminate the need for these compounds. (Note: You may try substituting tap water for distilled water. However, if you begin losing tissue sections, use only distilled water.) |
| Mount your tissue sections carefully. Tissue bonding begins quickly on Polysine™ slides. Tissue can be repositioned for approximately the first minute, but thereafter the bonding occurs rapidly and thoroughly. |
| Dry slides completely at room temperature by vertically draining them before heating them in an oven. A microwave oven can also be used for quick adhesion, once the tissue sections are thoroughly dried. |
SuperFrost® Plus GOLD Instruction for Use
| SuperFrost® Plus GOLD slides are inherently tissue binding so there is no need to add spray or adhesives to the slide. |
| Allow SuperFrost® Plus GOLD slide to contact the frozen tissue sections directly in the cryostat using your standard technique. |
| Place the slide horizontally on a table top (tissue side up) at room temperature for one minute. This allows time for the revolutionary surface binding chemistry in the SuperFrost® Plus GOLD slide to unite with the tissue section. This short time at room temperature does not cause frozen sections artifact because the SuperFrost® Plus GOLD slide holds both nuclear and cytoplasmic tissue components firmly immobilized in their place during the bonding process. The longer you allow for bonding time, the more the frozen tissue will adhere to the slide. |
| Postfix the SuperFrost® Plus GOLD slides if you wish, and stain as per your standard protocol. |
| Store the SuperFrost® Plus GOLD slides at room temperature. |
SuperFrost Ultra Plus® Instruction for Use
| Use your routine procedure for mounting cryostat sections (usually 3-10 µm thick), or for preparing cell smear / cytospin test samples. Float your 2-5 µm thick paraffin sections in a pre-heated water bath filled with distilled water. Do not add glue or subbing solution to the water bath or onto the glass. The Ultra Plus slide treatment eliminates the need to use these components. |
| Mount your sections carefully the first time, because tissue bonding begins quickly. Carefully dry with a fine absorbent paper. |
| Make sure your section is properly spread and there are no wrinkles to interfere with direct contact between the section and the glass surface |
| Dry slides on a hot plate ( 75-80°C ) for 15 min. to melt paraffin around sections. Cool slides to room temperature for immediate dewaxing and use for H & E, tinctorial staining or urgent immunohistochemistry using HIER. Alternatively: dry slides completely at room temperature by draining them vertically before heating them in an oven. |
| For optimum section adherence in HIER assisted immunohistochemistry, oven heating at 60°C for between 2h to 16h is required before dewaxing |
top